rabbit anti mouse jagged1  (Santa Cruz Biotechnology)

Journal: Molecular cell

Article Title: Systematic Characterization of Mutations Altering Protein Degradation in Human Cancers

doi: 10.1016/j.molcel.2021.01.020

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-human/mouse JAG1 , Cell Signaling Technology , Cat#70109.

Techniques: Control, Virus, Recombinant, In Vitro, Transfection, Western Blot, Capsules, Purification, Gel Extraction, cDNA Synthesis, RNA HS Assay, Plasmid Preparation, BIA-KA, Software, Adhesive

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Regulation of γδT17 cells by Mycobacterium vaccae through interference with Notch/Jagged1 signaling pathway

doi: 10.1590/1414-431X20209551

Figure Lengend Snippet: Expression of Jagged1 using immunohistochemical and immunofluorescence stains ( A ) in mice in control group ( a ), asthma group ( b ), gamma-secretase inhibitor (DAPT) + ovalbumin (OVA) group ( c ), Mycobacterium vaccae + OVA group ( d ), OVA + M. vaccae group ( e ), and its quantification ( B and C ). Data are reported as mean±SE relative percentage to the control group (n=8). ## P<0.01 vs control; **P <0.01 vs asthma group (ANVOA). Original magnification x400, scale bar=50 μm.

Article Snippet: Then, the sections were washed twice with PBS and incubated with an avidin D solution for 10 min. Endogenous peroxidase was blocked using a biotin solution to inhibit endogenous biotin, washed again, and subsequently incubated with rabbit anti-mouse Jagged1 antibody (1:1000; D4Y1R, CST) overnight at 4°C.

Techniques: Expressing, Immunohistochemical staining, Immunofluorescence, Control

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Regulation of γδT17 cells by Mycobacterium vaccae through interference with Notch/Jagged1 signaling pathway

doi: 10.1590/1414-431X20209551

Figure Lengend Snippet: The mRNA expression of Jagged1 in lung tissues was determined using real-time PCR. Data are reported as mean±SE relative percentage to the control group (n=8). *P<0.05 vs control; # P<0.05 vs asthma group (ANOVA). OVA: ovalbumin; DAPT: gamma-secretase inhibitor.

Article Snippet: Then, the sections were washed twice with PBS and incubated with an avidin D solution for 10 min. Endogenous peroxidase was blocked using a biotin solution to inhibit endogenous biotin, washed again, and subsequently incubated with rabbit anti-mouse Jagged1 antibody (1:1000; D4Y1R, CST) overnight at 4°C.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Regulation of γδT17 cells by Mycobacterium vaccae through interference with Notch/Jagged1 signaling pathway

doi: 10.1590/1414-431X20209551

Figure Lengend Snippet: Correlation analysis between Jagged1 and γδT17 cells in lung tissues. A and B , The ratio of IL-17+γδT+ cells to T cells was measured in each group. C , The percentage of IL-17+γδT+ cells and the Jagged1 mRNA expression in lung tissues were positively correlated (r=0.46, P<0.05). Data are reported as mean±SE relative percentage to the control group (n=8). **P<0.01 vs control; *P<0.05, ## P<0.01 vs asthma group (ANOVA). OVA: ovalbumin; DAPT: gamma-secretase inhibitor.

Article Snippet: Then, the sections were washed twice with PBS and incubated with an avidin D solution for 10 min. Endogenous peroxidase was blocked using a biotin solution to inhibit endogenous biotin, washed again, and subsequently incubated with rabbit anti-mouse Jagged1 antibody (1:1000; D4Y1R, CST) overnight at 4°C.

Techniques: Expressing, Control

Journal: Theranostics

Article Title: Serelaxin alleviates cardiac fibrosis through inhibiting endothelial-to-mesenchymal transition via RXFP1

doi: 10.7150/thno.38640

Figure Lengend Snippet: Serelaxin promotes the preservation of endothelial cell properties in both in vivo and in vitro by regulating candidate genes. (A) Heat map and (B) scatter plot show the genes with altered mRNA expression level in only TGFβ1-treated (x-axis) or in both TGFβ1- and Serelaxin-treated (y-axis) HCAECs. STEAP1, NOTCH1, JAGGED1, RAC1, DSP, GSC, ERBB3, FZD7, GSK3B and TGFB2 were significantly regulated by Serelaxin treatment, which is shown by separation from dot lines (cut-off by 4 folds). (C-D) qPCR analysis showing the expression of candidate genes, which were identified from qPCR array in TGFβ1- and Serelaxin-treated MCECs. Cells without any treatment were used as control. (E) qPCR analysis of the panel of candidate genes in vivo in sham and AAC-operated mice treated with vehicle or Serelaxin. Serelaxin significantly rescued the effects of AAC on all genes except for Gsc and Steap1. Student t-test was used for single comparison and one-way ANOVA with Bonferroni post-hoc analysis was used for multiple group comparisons. Gene expression and associated error bars, representing mean ± SEM n≥3, n.s. no significance, * p<0.05, ** p<0.01, *** p<0.001.

Article Snippet: Rabbit Anti-Mouse Jagged1 , 1:50 , Santa Cruz , SC8303.

Techniques: Preserving, In Vivo, In Vitro, Expressing, Control, Comparison, Gene Expression

Journal: Theranostics

Article Title: Serelaxin alleviates cardiac fibrosis through inhibiting endothelial-to-mesenchymal transition via RXFP1

doi: 10.7150/thno.38640

Figure Lengend Snippet: Serelaxin rescues the Notch1 pathway in AAC-operated hearts. Immunofluorescence staining of (A) Jagged1, (B) Notch1 and (C) NICD in combination with WGA and DAPI in sham and AAC-operated hearts treated with vehicle or Serelaxin. Quantifications showed that protein expressions of (D) Jagged1, (E) Notch1 and (F) NICD were downregulated in AAC-operated hearts and could be restored by Serelaxin. (G) Western blot analysis shows the amount of the soluble form of Jagged1 in the medium of TGFβ1-treated MCECs. Ponceau-S stained membrane picture indicates that an equal amount of total precipitated protein was loaded for both the control and TGFβ1-treated MCECs. Soluble Jagged1 was reduced in TGFβ1-treated MCECs as quantified by densitometry analysis (right panel). Student t-test was used for single comparison and one-way ANOVA with Bonferroni post-hoc analysis was used for multiple group comparisons. Gene expression and associated error bars, representing mean ± SEM, n≥3, n.s. no significance, * p<0.05, ** p<0.01, *** p<0.001.

Article Snippet: Rabbit Anti-Mouse Jagged1 , 1:50 , Santa Cruz , SC8303.

Techniques: Immunofluorescence, Staining, Western Blot, Membrane, Control, Comparison, Gene Expression

Journal: Theranostics

Article Title: Serelaxin alleviates cardiac fibrosis through inhibiting endothelial-to-mesenchymal transition via RXFP1

doi: 10.7150/thno.38640

Figure Lengend Snippet: Antibodies used in Immunofluorescence staining

Article Snippet: Rabbit Anti-Mouse Jagged1 , 1:50 , Santa Cruz , SC8303.

Techniques: Immunofluorescence

Journal: Theranostics

Article Title: Serelaxin alleviates cardiac fibrosis through inhibiting endothelial-to-mesenchymal transition via RXFP1

doi: 10.7150/thno.38640

Figure Lengend Snippet: Primers used in qPCR assay for gene expression analysis

Article Snippet: Rabbit Anti-Mouse Jagged1 , 1:50 , Santa Cruz , SC8303.

Techniques: Gene Expression, Sequencing

Journal: Theranostics

Article Title: Serelaxin alleviates cardiac fibrosis through inhibiting endothelial-to-mesenchymal transition via RXFP1

doi: 10.7150/thno.38640

Figure Lengend Snippet: Antibodies used in Immunofluorescence staining

Article Snippet: Rabbit Anti-Mouse Jagged1 , 1:50 , Santa Cruz , SC8303.

Techniques: Immunofluorescence